Thermo Fisher Scientific ZO-1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 85 publications 85 publications

Immunohistochemistry (IHC)

-

View 96 publications 96 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

2.5-5 µg/mL

View 122 publications 122 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Chicken, Dog, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide in the middle region of the ZO-1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533456

Product Specific Information

40-2200 was used successfully in the immunofluorescence analysis of Zo-1 in MDCK cells.

Target Information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.