Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공됩니다. 세포 간 접합 및 신호전달 연구에 유용합니다.
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Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 85 publications |
| Immunohistochemistry (IHC) | - | View 96 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 3 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View 9 publications |
| Immunocytochemistry (ICC/IF) | 2.5–5 µg/mL | View 122 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | - | View 2 publications |
| Miscellaneous PubMed (Misc) | - | View 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Bovine, Chicken, Dog, Human, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide in the middle region of the ZO-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533456 |
Product Specific Information
Antibody 40-2200 was successfully used in the immunofluorescence analysis of ZO-1 in MDCK cells.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins associate with claudin, occludin, and F-actin at tight junction strands, providing linkage between the actin cytoskeleton and tight junctions.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, whereas ZO-1 is also expressed at cell junctions of cardiac myocytes.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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