Thermo Fisher Scientific PUMA delta/gamma Polyclonal Antibody
PUMA delta/gamma 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot에 최적화되어 있으며 Human 및 Mouse 시료에 반응. Protein G 정제 및 PBS/BSA 완충액에 보관. p53 경로 관련 세포사멸 연구용.
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Applications
- Western Blot (WB): 2–5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36–53 of human PUMA delta and amino acids 196–213 of human PUMA gamma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1960159 |
Product Specific Information
PA1-29807 detects Puma gamma from human samples.
The amino acid sequence used as immunogen has 100% identity in PUMA delta and PUMA gamma, and 59% identity in PUMA alpha.
Target Information
PUMA is one of the pro-apoptotic Bcl-2 family members, including Bax and Noxa, which are also transcriptional targets of p53.
The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b.
PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53.
PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduces the apoptotic response to p53, suggesting PUMA plays a key role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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