Thermo Fisher Scientific MYH8 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

• Detects endogenous MYH8 protein at a molecular weight of 223 kDa.
• Purity: >95% by SDS-PAGE.

Target Information

Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits, and a pair of phosphorylatable light chain subunits.
Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins, and non-muscle Myosins.

Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase.
Myosin heavy chains, encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin.

Myosin heavy chains were initially isolated from human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle.
Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.