Takara Efficient generation of edited hiPS clonal populations

Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. TheCellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kitis a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.

Establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time-consuming; often, it results in cell death or premature differentiation. TheCellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kitis a complete system for efficient expansion and scale-up manufacturing of hiPS cells in a feeder-free and defined environment. The kit contains all the necessary reagents to go from seeding single hiPS cells into 96-well plates to expansion of clones into 48-well plates. Following clonal expansion, hiPS cells can continue to be cultured using the Cellartis DEF-CS 500 Culture System.

To generate your own edited clonal hiPS cell lines from start to finish, an electroporation-based or gesicle-based Cas9-sgRNA RNP complex delivery system can be combined with the Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit. Please refer to the following technotes to learn more:

• Editing iPS cells using electroporation »
• Editing iPS cells using gesicle technology »

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Overview

• Superior single-cell survival—edited hiPS cells exhibit high (typically ~50%) survival when seeded as single cells in a 96-well plate
• Maintenance of pluripotency during single-cell cloning—hiPS cells maintain high levels (>90%) of pluripotency markers Oct-4, TRA-1-60, and SSEA-4
• Stable karyotype from start to finish—hiPS cells maintain a normal and stable karyotype throughout single-cell cloning and expansion
• CRISPR/Cas9 gene-editing solutions available—use our electroporation-based or gesicle-based CRISPR/Cas9 techniques prior to single-cell cloning for a start-to-finish solution for generating clonal hiPS cell lines

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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