Thermo Fisher Scientific Phospho-C/EBP epsilon (Ser181) Polyclonal Antibody
C/EBP epsilon 단백질의 Ser181 인산화 형태를 특이적으로 검출하는 폴리클로날 항체. Western blot 및 IHC(파라핀) 응용에 적합. 인간, 생쥐, 랫트 반응성. 고순도 정제 및 안정한 PBS/glycerol 저장용액으로 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CEBPE (Accession Q15744), corresponding to amino acid residues around phosphorylated Ser181 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817085 |
Product Specific Information
Antibody detects endogenous levels of C/EBP epsilon only when phosphorylated at Ser181.
Target Information
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine.
C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper.
Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces.
C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells.
Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta, and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha.
Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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