Thermo Fisher Scientific MAPKAPK5 Monoclonal Antibody (OTI4G4), TrueMAB
MAPKAPK5 단백질을 인식하는 Mouse monoclonal antibody로, Western blot에 최적화됨. Human 시료 반응성, carrier-free 형태로 재결합 및 conjugation 실험에 적합. Lyophilized 형태로 안정적이며 -20°C에서 보관 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 197–471 of human MAPKAPK5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPKAPK5 (also known as PRAK) is a serine/threonine kinase activated in response to cellular stress and pro-inflammatory cytokines. Its activity is regulated by p38 alpha and p38 beta MAP kinases. This kinase phosphorylates heat shock protein HSP27 at physiologically relevant sites. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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