Thermo Fisher Scientific MAPKAPK5 Monoclonal Antibody (OTI1B3), TrueMAB
인간 MAPKAPK5 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot에 최적화되어 있습니다. 동결건조 형태로 제공되며, PBS와 trehalose 버퍼에 저장됩니다. 연구용으로 스트레스 반응 및 염증성 신호전달 연구에 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 197–471 of human MAPKAPK5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Its activity is regulated by p38 alpha and p38 beta. The PRAK protein belongs to the serine/threonine kinase family and phosphorylates HSP27 at physiologically relevant sites. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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