Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• FITC
• PE and PE derivatives
• PerCP-Cyanine5.5
• Super Bright series
• NovaFluor Blue / Red / Yellow variants
• Functional Grade
• Custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤1.0 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test)

It is recommended to titrate the antibody for optimal performance.
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining. Add 5 µL per sample to antibody cocktails or single-color controls.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates surface expression of the TCR complex. Subunits CD3 gamma, delta, epsilon, and zeta are members of the immunoglobulin superfamily encoded on chromosome 11. CD3 is expressed on most T cells and thymocytes but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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