Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), NovaFluor Yellow 590, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-70S, 660-120S
• NovaFluor Red 685, 700, 710
• NovaFluor Yellow 610, 660, 690, 700, 730
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 780
• Request custom conjugation available

Product Specific Information

Description:
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. Commonly used as a phenotypic marker for mouse T cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1.0 µg/test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Additional Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Include CellBlox Blocking Buffer when staining with NovaFluor conjugates (5 µL per sample).
• Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow).
• NovaFluor conjugates use Phiton technology with engineered fluorescent signatures minimizing spillover and spread.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are members of the immunoglobulin superfamily encoded on chromosome 11. They associate with cytoplasmic signaling molecules via tyrosine-based motifs. CD3 is present on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells. Altered CD3 expression may occur in autoimmune diseases. CD3 gene defects are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

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