Thermo Fisher Scientific NFkB p65 Polyclonal Antibody, eBioscience
NFkB p65 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat에 반응합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, PBS 완충액에 보관됩니다. 세포 내 염증 반응 및 전사 조절 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | 12 |
| Immunohistochemistry (IHC) | 1:1,000 | 4 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 1:100 | 4 |
| Immunoprecipitation (IP) | Assay-Dependent | – |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | 1 |
| Immunomicroscopy (IM) | – | 1 |
| Gel Shift (GS) | – | 1 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.02% gelatin, BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468254 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The polyclonal antibody reacts with mouse, rat, and human NFkB p65; it was raised against a peptide mapping to the carboxy terminus of human NFkB p65. Members of the Rel/NFkB family of transcription factors regulate cellular processes such as growth, development, and inflammation. They contain a Rel homology region (RHR) mediating protein dimerization. Complexes of p50 (NF-kB1) or p52 (NF-kB2) associate with Rel family members (p65, c-Rel, RelB) to regulate transcription of different genes.
In resting cells, NFkB is retained in the cytoplasm by IkB proteins. Upon activation by cytokines, LPS, or oxidative stress, IkB is degraded, allowing NFkB to translocate to the nucleus and activate proinflammatory genes. NFkB activation is implicated in various pathological conditions, including cardiac ischemia-reperfusion injury and insulin-dependent diabetes mellitus.
Applications Reported: Suitable for immunoprecipitation, Western blotting, and immunohistochemistry.
Applications Tested: Tested in WB and IHC (recommended starting dilution 1:1000). Optimize dilution for specific assays.
Target Information
Nuclear factor kB p65 (NF-kB p65), encoded by the RELA gene on human chromosome 11, is also known as RelA. It forms heterodimers with NF-kB subunits p50 or p52 and plays a key role in the transcriptional regulation of immune and inflammatory genes. Activation occurs via TNFα-induced IKK phosphorylation, leading to IκB degradation and nuclear translocation of NFkB. It is critical for immunoglobulin κ gene transcription in mature B lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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