Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 436, eBioscience
인간 CD14 항원을 인식하는 61D3 클론 단일클론 항체로, Super Bright 436 형광체로 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 436 nm에서 방출됩니다. 고순도 친화 크로마토그래피 정제 및 안정적인 PBS 버퍼 저장.
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Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 436, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 23 publications |
| Neutralization (Neu) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 61D3 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2724060 |
Product Specific Information
The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. CD14 binds LPS-LBP complexes with high affinity on monocyte surfaces.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells
- Recommended Use: 5 µL (0.5 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test)
- Fluorochrome Details: Super Bright 436 excited at 405 nm, emits at 436 nm; use a 450/50 bandpass filter or equivalent
- Buffer Recommendation: Use Super Bright Complete Staining Buffer (Product # SB-4401) when multiple Super Bright dyes are used to minimize polymer interactions
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 participates in immune responses, apoptotic cell binding, and inflammatory processes.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.
Available Conjugate Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–786), eFluor series (450, 506), FITC, Functional Grade, NovaFluor series (Blue, Yellow), PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright series (600–780), and custom conjugation available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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