Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Super Bright 436, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–711)
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue and Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 780
• Request custom conjugation

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c associates with beta 2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells and some activated T cells. It binds to CD54, iC3b, and fibrinogen, contributing to leukocyte adhesion.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
• Excitation/Emission: 405 nm / 436 nm (Violet laser)
• Recommended filter: 450/50 bandpass or equivalent
• Buffer recommendation: Use Super Bright Complete Staining Buffer (SB-4401) when combining multiple Super Bright conjugates.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11, along with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes.
• CD11c: Found in monocytes, macrophages, NK cells, some granulocytes, and a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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