Thermo Fisher Scientific ATF5 Recombinant Rabbit Monoclonal Antibody (SD2099)
ATF5 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체(SD2099). Western blot, IHC, ICC, Flow cytometry에 사용 가능. 높은 특이성과 일관된 로트 간 품질 제공. 단백질 발현 및 세포 생존 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:1,000 | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD2099 |
| Immunogen | Synthetic peptide within Human ATF5 aa 203–252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809646 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
ATF5 is a protein-coding gene functioning as a transcription factor that can stimulate or repress gene transcription through binding to DNA regulatory elements such as cAMP response element (CRE), ATF5-specific response element (ARE), and amino acid response element (AARE).
It plays critical roles in:
- Cell survival, proliferation, and differentiation (cell type-dependent)
- Cerebral cortex formation and neuroprogenitor cell regulation
- Olfactory sensory neuron survival
- Osteogenic differentiation
- Regulation of BCL2 and EGR1 expression (anti-apoptotic function)
- Cooperation with NR1I3/CAR in CYP2B6 transcriptional activation in liver
- Regulation of centrosome dynamics and adipocyte differentiation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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