Thermo Fisher Scientific ATF4 Recombinant Rabbit Monoclonal Antibody (SD20-92)
ATF4 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. 재조합 시스템으로 생산되어 높은 특이성과 재현성을 제공합니다. Human, Mouse, Rat 시료에 반응하며, 단백질 A로 정제된 액상 형태입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD20-92 |
| Immunogen | Recombinant protein within Human ATF4 aa 1–220 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809645 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The ATF4 gene encodes a transcription factor originally identified as a mammalian DNA-binding protein that interacts with the tax-responsive enhancer element in HTLV-1 LTR. It is also known as CREB-2 (cAMP-response element binding protein 2).
This protein belongs to a family of DNA-binding proteins including AP-1, CREBs, and CREB-like proteins. These transcription factors share a leucine zipper region for protein-protein interactions and a basic DNA-binding domain.
Two alternative transcripts encoding the same protein have been described, and two pseudogenes are located on the X chromosome at q28 within an inverted duplication region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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