
Thermo Fisher Scientific ERBB2 Monoclonal Antibody (OTI4F10), TrueMAB
Human ERBB2 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 676–1255 of human ERBB2 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ErbB2 (HER2) is a receptor tyrosine kinase overexpressed in some breast tumors. Herceptin, used in treatment of metastatic HER2-positive cancer, is a monoclonal antibody targeting this kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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