Thermo Fisher Scientific CYP17A1 Monoclonal Antibody (OTI3F11), TrueMAB
인간 CYP17A1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, Flow Cytometry에 최적화됨. 무보존 Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 약물 대사 및 스테로이드 합성 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F11 |
| Immunogen | Full length human recombinant protein of human CYP17A1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CYP17A1 encodes a cytochrome P450 enzyme involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
It localizes to the endoplasmic reticulum and exhibits both 17α-hydroxylase and 17,20-lyase activities, playing a key role in steroidogenic pathways producing progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens.
Mutations are associated with steroid-17α-hydroxylase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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