Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 725, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV/Violet/Yellow series
• PE, PE-Cyanine5/5.5/7, PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436~780
• NovaFluor Blue, Red, UV, Violet, Yellow series
• Request custom conjugation available

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds to MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes ≤0.2 µg/test (100 µL final volume)

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread impacts.

Excitation: 493 nm
Emission: 720 nm
Laser: 488 nm (Blue)

Target Information

CD8 is a type I transmembrane glycoprotein of the immunoglobulin family, functioning as a co-receptor with TCR for MHC-I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8 engagement with MHC-I/peptide complexes activates Lck kinase, promoting lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activity. CTL differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

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