Thermo Fisher Scientific PAK3 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Positive test controls include: U-251MG, Rat brain, Rat testis.
• Immunogen sequence:
  MSDGLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTGIPEQWAR

Target Information

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling.
PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.
The alpha, beta, and gamma PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation.
There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141, and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation.
Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.