Thermo Fisher Scientific HSP90 alpha Monoclonal Antibody (3B6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Non-human primate, Rabbit

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B6

Immunogen

Purified mouse HSP90.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325458

Product Specific Information

MA3-010 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, and bovine tissues. This antibody does not detect chicken HSP90.

MA3-010 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired. MA3-010 does not immunoprecipitate HSP90 in any form (free or complexed).

The MA3-010 antigen is purified mouse HSP90.

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.