Thermo Fisher Scientific HSP70 Monoclonal Antibody (4G4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Flow Cytometry (Flow)

1:100 - 1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

1:1,000

-

Neutralization (Neu)

-

View 3 publications 3 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Inhibition Assays (IA)

Assay-dependent

-

Cytotoxicity Assay (CT)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4G4

Immunogen

Human recombinant HSP70 over expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325457

Product Specific Information

MA3-009 detects heat shock protein 70 kDa (HSP70) from mouse and human tissues. This antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family.

MA3-009 has been successfully used in Western blot, IF, blocking, FACS, inhibition assay, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 70 kDa protein representing HSP70 from K562 cell extract.

The MA3-009 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

Target Information

HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.