Thermo Fisher Scientific HSP70 Monoclonal Antibody (4G4)
HSP70 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 단클론 항체(4G4)로, 인간 및 마우스 시료에서 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, 70 kDa HSP70 단백질을 검출합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 15 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 | – |
| Immunocytochemistry (ICC/IF) | 1:100 | View 2 publications |
| Flow Cytometry (Flow) | 1:100–1:200 | View 2 publications |
| Immunoprecipitation (IP) | 1:1,000 | – |
| Neutralization (Neu) | – | View 3 publications |
| Immunomicroscopy (IM) | – | View 1 publication |
| Inhibition Assays (IA) | Assay-dependent | – |
| Cytotoxicity Assay (CT) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G4 |
| Immunogen | Human recombinant HSP70 overexpressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | PBS, ascites |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325457 |
Product Specific Information
MA3-009 detects heat shock protein 70 kDa (HSP70) from mouse and human tissues.
This antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family.
It has been successfully used in Western blot, immunofluorescence, blocking, FACS, inhibition assay, immunohistochemistry, and immunoprecipitation procedures.
By Western blot, this antibody detects a 70 kDa protein representing HSP70 from K562 cell extract.
The MA3-009 antigen is recombinant human HSP70 overexpressed in E. coli.
Epitope mapping with a panel of HSP70 deletion mutants suggests that the recognized epitope is located between amino acids 504–617 of human HSP70, a region involved in stress-induced nucleolar localization.
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including high temperatures.
The HSP70 family is a group of highly conserved proteins induced by heat stress in all organisms studied.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70: Constitutively expressed; involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): Glucose-regulated 78 kDa protein localized in the endoplasmic reticulum
- p75 (HSP75): 75 kDa mitochondrial protein
HSP70 is encoded by an intronless gene and, along with other heat shock proteins, stabilizes existing proteins against aggregation and assists in folding newly translated proteins in the cytosol and organelles.
It is also involved in the ubiquitin-proteasome pathway through interaction with AU-rich element RNA-binding protein 1.
The HSP70 gene is located in the major histocompatibility complex class III region, clustered with two closely related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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