Thermo Fisher Scientific CD44/HCAM Std. Recombinant Mouse Monoclonal Antibody (rHCAM, 918)
CD44/HCAM 표면 항원을 인식하는 Thermo Fisher Scientific의 Mouse Recombinant Monoclonal Antibody입니다. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. HEK293 발현 시스템 기반, Protein A/G 정제, 무보존액 PBS 용액 형태로 제공됩니다.
- 카탈로그번호
- 960-MSM20-P1ABX
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- pk
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD44/HCAM Std. Recombinant Mouse Monoclonal Antibody (rHCAM, 918)
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Baboon, Human, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rHCAM, 918 |
| Immunogen | Recombinant full-length human CD44 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
CD44 cell surface antigen is a 100 kDa type I transmembrane glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and plays roles in cell-cell interactions, adhesion, and migration.
It participates in lymphocyte activation, recirculation, and homing. CD44 expression may be upregulated in certain carcinomas, potentially linked to metastatic potential.
CD44 is expressed by hematopoietic and non-hematopoietic cells, epithelial tissues, and filopodia in cultured keratinocytes. Bone marrow myeloid cells and memory T cells express CD44 at high levels, while peripheral B and T cells can upregulate expression upon stimulation.
Alternative splicing of CD44 transcripts results in multiple isoforms with distinct functions. Disorders associated with CD44 dysfunction include superficial keratitis and lichen sclerosus, and CD44 may be related to tumor metastasis formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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