Thermo Fisher Scientific F(ab`)2-Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP
Western blot, IHC, ELISA에 적합한 HRP 결합 F(ab’)2 형태의 Donkey anti-Mouse IgG(H+L) 2차 항체. 높은 교차흡착으로 비특이적 결합 최소화. 친화 크로마토그래피 정제 및 안정한 PBS/BSA 버퍼 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535975 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep IgG |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target antibodies. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may undergo further purification or modification such as immunoaffinity chromatography, fragmentation, or label conjugation to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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