Thermo Fisher Scientific F(ab`)2-Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 결합된 Donkey anti-Mouse IgG (H+L) F(ab')₂ 단편 항체로, 면역세포염색(ICC/IF)에 적합합니다. 높은 교차흡착으로 비특이적 결합을 최소화하며, 552/578 nm의 형광 특성을 가집니다. 연구용으로 높은 신뢰성과 재현성을 제공합니다.
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Applications
- Immunocytochemistry (ICC/IF): 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552/578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535980 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep IgG |
| Antibody Form | F(ab`)₂ Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product is overfilled to ensure complete recovery.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Shelf life: 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum proteins or IgG from other listed species.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse antibodies.
Secondary antibodies provide versatility and increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These reagents are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 647, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- AP, Biotin, FITC, HRP
- Custom conjugation available upon request
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