Thermo Fisher Scientific F(ab`)2-Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody
마우스 IgG(H+L)에 특이적인 F(ab')2 형태의 고순도 2차 항체. 당나귀 유래 다클론 항체로, 높은 교차흡착 처리로 비특이적 반응 최소화. ELISA 등 다양한 면역검출 실험에 적합. 비결합(Unconjugated) 상태로 제공.
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Applications
Tested Dilution
- ELISA: 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535977 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep IgG |
| Antibody Form | F(ab`)2 Fragment |
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide increased versatility, allowing use with various detection systems (e.g., HRP, AP, fluorescence), and greater sensitivity via signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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