Thermo Fisher Scientific PRAS40 Polyclonal Antibody, Biotin
PRAS40 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal 항체입니다. Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능합니다. Affinity chromatography로 정제되었으며, -20°C에서 보관합니다. Human, Mouse, Rat 시료에 반응합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 160–210 on human Proline-rich AKT1 substrate 1 isoform a protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after various cell death signals. AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. It is also a substrate and potential regulator of mTOR, which controls cell growth and cycle, and acts as a negative regulator of autophagy. Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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