Thermo Fisher Scientific Rat IFN-alpha Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Rat

Expression System

Insect cells

Molecular weight

19 kDa

Class

Recombinant

Type

Protein

Endotoxin concentration

≤1 EU/vial

Activity

Specific Activity = 1x10e8 units/mg; one unit of activity is the amount of recombinant rat IFN-alpha that inhibits the cytopathic effect of VSV by 50% in monolayer cultures of Ratec cells grown in 96-well microtiter plates. Important Note: This recombinant rat IFN-alpha requires the presence of a reducing agent such as glutathione for full biological activity.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

citric acid, pH 5, with 125mM trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Reconstitution: Dissolve the contents of the vial by injection of 150 µL sterile distilled water. Lyophilized product is stable for a defined length of time at -20°C. After reconstitution, the contents can be best divided into small aliquots for single use and stored at -80°C. After thawing, the cytokine is stable for at least four weeks at 4°C

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.