Thermo Fisher Scientific Rat IFN-alpha Recombinant Protein
Rat 유래 IFN-alpha 재조합 단백질로, 항바이러스 면역 연구에 활용 가능. Insect cell 발현, 19 kDa 크기, 동결건조 형태. ELISA, WB, IHC 등 다양한 실험에 사용 가능. 완전 활성 위해 환원제 필요.
- 카탈로그번호
- PRC4014
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Functional Assay (Functional): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Rat |
| Expression System | Insect cells |
| Molecular Weight | 19 kDa |
| Class | Recombinant |
| Type | Protein |
| Endotoxin Concentration | ≤1 EU/vial |
| Activity | Specific Activity = 1×10⁸ units/mg; one unit of activity is the amount of recombinant rat IFN-alpha that inhibits the cytopathic effect of VSV by 50% in monolayer cultures of Ratec cells grown in 96-well microtiter plates. Note: This recombinant rat IFN-alpha requires the presence of a reducing agent such as glutathione for full biological activity. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | Citric acid, pH 5, with 125 mM trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Reconstitution:
Dissolve the contents of the vial by injection of 150 µL sterile distilled water.
Lyophilized product is stable for a defined period at -20°C.
After reconstitution, divide into small aliquots for single use and store at -80°C.
After thawing, the cytokine is stable for at least four weeks at 4°C.
Target Information
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates.
The interaction of IFN-alpha with its receptor components activates multiple signaling pathways involved in innate immune responses against viral infection.
In humans, the IFN alpha gene is located on chromosome 9.
Type I interferons include 14 alpha isoforms and single beta, omega, epsilon, and kappa isoforms.
Homologous molecules exist in many species, including rats, mice, birds, reptiles, and fish.
Produced mainly by plasmacytoid dendritic cells, IFN alpha is a potent component of the antiviral innate immune response and modulates adaptive immunity.
Dysfunction of IFN alpha is associated with diseases such as Hepatitis C and Hepatitis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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