Thermo Fisher Scientific AFP Polyclonal Antibody
AFP 단백질을 인식하는 Rabbit Polyclonal 항체로 Western Blot, IHC, ICC에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로 간편한 액상 형태로 제공.
- 카탈로그번호
- PA521004
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 2.5–10 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 14 amino acid synthetic peptide near the center of human AFP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157055 |
Product Specific Information
- Suggested positive control: human liver tissue lysate
- PA5-21004 can be used with blocking peptide PEP-1118
- The immunogen is located within amino acids 340–390 of AFP
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal life.
AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also occur without pathology.
AFP is considered the fetal counterpart of serum albumin, and both genes are located in tandem on chromosome 4.
AFP exists in monomeric, dimeric, and trimeric forms and can bind to copper ions, nickel ions, fatty acids, and bilirubin.
AFP levels in amniotic fluid are used to detect neural tube defects such as spina bifida and anencephaly.
Elevated serum AFP levels are observed in hepatocellular carcinoma (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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