Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody, DyLight 800
FLAG(DYKDDDDK) 태그 검출용 DyLight 800 결합 폴리클로날 항체로, WB, ICC/IF, ELISA에 적합. 고순도 친화성 정제 제품으로 높은 감도와 특이성을 제공. 단백질 발현 및 정제 연구에 활용 가능.
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Thermo Fisher Scientific DYKDDDDK Polyclonal Antibody, DyLight 800
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:10,000–1:25,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody from rabbit serum immunized with Enterokinase Cleavage Site peptide (D-Y-K-D-D-D-D-K) conjugated to KLH. Reacts with FLAG™ conjugated proteins. |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to use.
This affinity purified antibody is directed against the FLAG epitope tag and is useful for detecting FLAG-tagged proteins in various assays. It recognizes the FLAG epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the N- or C-terminus of target proteins in transfected or transformed cells.
Target Information
DYKDDDDK tag (FLAG tag) is a short peptide sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) used as a protein tag for purification, detection, and localization. It can be fused to the N- or C-terminus of a protein, enabling easy identification using anti-FLAG antibodies. Widely used in molecular biology and biochemistry research for protein-protein interaction, expression, and localization studies. The FLAG tag is hydrophilic, minimizing interference with protein function. FLAG™ is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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