Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI1D11), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and participates in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and tumor tissue prognosis, localized in the nucleus, acting as a cofactor of DNA polymerase delta. It functions as a homotrimer to enhance leading strand synthesis during DNA replication.
Upon DNA damage, PCNA is ubiquitinated and participates in RAD6-dependent DNA repair. Two transcript variants encode the same protein, and pseudogenes exist on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.