Thermo Fisher Scientific Synaptotagmin 1 Monoclonal Antibody (ASV48)
Synaptotagmin 1 단백질을 표적하는 마우스 단클론 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 단백질 G로 정제된 액상 항체이며, 1.0 mg/mL 농도로 제공됩니다. 신경전달물질 방출 및 시냅스 소포 기능 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:500 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Mouse, Non-human primate, Rat, Shark |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ASV48 |
| Immunogen | Rat brain synaptic junction protein complexes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2, with 0.1 mM PMSF, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_795476 |
Product Specific Information
Possible positive controls for this product are rat brain tissue extract.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein widely expressed throughout the CNS, functioning as the Ca²⁺ sensor in exocytosis and neurotransmitter release. Its Ca²⁺-mediated binding of SNAP25 is essential for Ca²⁺-dependent membrane fusion. Specific residues within the C2B domain influence endocytic rate and vesicle size. Synaptotagmin 1 regulates synaptic vesicle trafficking at the active zone and binds acidic phospholipids requiring both acidic head groups and diacyl backbones. It also interacts with neurexins, syntaxin, and AP2 in a Ca²⁺-independent manner. Dysfunctions are associated with food-borne and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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