Thermo Fisher Scientific PRMT5 Monoclonal Antibody (PRMT5-21)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PRMT5-21

Immunogen

Fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (residues 315-637). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PRMT5,uniProtId:O14744-1,ncbiNodeId:9606,antigenRange:315-637,antigenLength:637,antigenImageFileName:MA1-25470_PRMT5_O14744-1_House_mouse.svg,antigenImageFileNamePDP:MA1-25470_PRMT5_O14744-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.4 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2253071

Product Specific Information

Recommended positive controls: G361.

Target Information

PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.