Thermo Fisher Scientific Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody (4S5Y7)
Cytokeratin 10 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포에서 발현되며, 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4S5Y7 |
| Immunogen | Synthetic peptide corresponding to amino acids 485–584 of human Cytokeratin 10 (UniProt ID: P13645) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911999 |
Product Specific Information
- Positive test controls: HeLa, A-431
- Immunogen sequence:
GGHGGGHGGS SGGGYGGGSS GGGSSGGGYG GGSSSGGHGG SSSGGYGGGS SGGGGGGYGG GSSGGGSSSG GGYGGGSSSG GHKSSSSGSV GESSSKGPRY
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, found in both keratinizing (e.g., skin) and non-keratinizing tissues (e.g., mesothelial cells). Although not typical markers for endothelial cells, some microvascular endothelial cells also express cytokeratins.
There are at least 20 cytokeratin subtypes ranging from 40–70 kDa with isoelectric points of 5–8.5. CKs are classified into two types:
- Type I: acidic polypeptides
- Type II: basic polypeptides
Each epithelial cell expresses at least one member of each type. Monoclonal antibodies to cytokeratins are useful for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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