Thermo Fisher Scientific MCPT-1 Recombinant Rabbit Monoclonal Antibody (43)

Applications

ELISA (ELISA)

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

This antibody has specificity for Mouse MCPT-1/MCP-1.

Target Information

MCPT-1 is the only chymase expressed by intestinal mucosal mast cells, which are found in the intestinal epithelium. Although it is expressed constitutively and detectable in the sera of normal mice, intestinal parasites cause systemic levels to increase dramatically within two days and peak at two weeks following infection.

It plays an important role in host defense against intestinal parasites, as mice deficient in MCPT-1 display delayed responses upon infection. Elevated MCPT-1 levels are also observed during intestinal allergic hypersensitivity reactions. The mechanism of its action is not fully understood, but it is believed to increase intestinal permeability similarly to rMCPT-II, possibly by cleaving proteins at tight junctions between cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.