Thermo Fisher Scientific MiTF Polyclonal Antibody
MiTF 단백질을 검출하기 위한 Rabbit Polyclonal 항체로, Western blot, IHC, ICC에 적합합니다. Human, Mouse, 비인간 영장류 반응성을 가지며, 고순도의 Affinity chromatography로 정제되었습니다. PBS/glycerol buffer에 보관되며 -20°C에서 안정적으로 저장 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MITF (Accession O75030), corresponding to amino acid residues Q154–R204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818432 |
Product Specific Information
This antibody detects endogenous levels of total MITF.
Target Information
Mi (Microphthalmia-associated transcription factor, MITF) is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor involved in pigmentation, mast cell, and bone development. Mutations in Mi cause Waardenburg Syndrome type II in humans. In mice, loss of Mi results in depigmentation of skin, eye, and inner ear, as well as osteopetrosis and defects in natural killer and mast cells.
Two isoforms exist, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes (52–56 kDa), while longer forms (60–70 kDa) are found in osteoclasts, B16 melanoma cells, mast cells, and heart tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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