Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Super Bright 702, eBioscience

Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Super Bright 702, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test
• Publications: View 9 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 395 / 615 / 661 / 737
• Brilliant Violet 605 / 650 / 711
• FITC
• Functional Grade
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600
• Request custom conjugation

Product Specific Information

Description:
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages, dendritic cells, and activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a critical role in T-B cell communication and activation.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-diluted and tested on Daudi cell line for flow cytometry. Recommended usage: 5 µL (1.0 µg) per test (100 µL volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Recommended filter: 710/50 bandpass.

Staining Recommendations:
For panels with multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer ([Product # 00-8222-49])
• 1-step Fix/Lyse Solution ([Product # 00-5333-57])
  Clone-specific performance should be validated empirically.

Excitation / Emission / Laser:

• Excitation: 405 nm
• Emission: 702 nm
• Laser: Violet Laser

Licensing:
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation.
CD80 expression is rapidly induced in activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma, T cells, and monocytes.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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