Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), Super Bright 702, eBioscience

Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), Super Bright 702, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 7 publications

Product Specifications

Available Formats

• Unconjugated
• eFluor 450
• Functional Grade
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

eBioTY/11.8 recognizes CD73, a 69 kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity.
CD73 expression on myeloid cells (CD11b) is restricted to bone marrow. In humans, CD73 can be induced to secrete a soluble form with IL-2, suggesting a role in activation signaling.

CD73 is expressed on subsets of lymphocytes and increases during maturation. It is found on memory CD4 T cells and regulatory T cells, indicating roles in immune modulation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes, ≤ 0.5 µg/test (in 100 µL volume). Cell count: 10⁵–10⁸ cells/test. Titration recommended for optimal performance.

Super Bright 702 is a tandem dye excited by a 405 nm violet laser and emits at 702 nm. Recommended filter: 710/50 bandpass.
Use Super Bright Complete Staining Buffer (SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in dark.
Excitation: 405 nm | Emission: 702 nm | Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD73 (Ecto-5'-nucleotidase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, preferentially AMP.
It forms a dimer of two identical 70 kDa subunits anchored to the plasma membrane via GPI linkage.
CD73 serves as a marker of lymphocyte differentiation, and its deficiency is associated with various immunodeficiency diseases.
The CD73 gene is localized to chromosome 6q14–q21. Defects can lead to joint and arterial calcification and intestinal tuberculosis.
Two transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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