Thermo Fisher Scientific p300 Monoclonal Antibody (RW128)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:500

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

2-5 µg/mL

-

Flow Cytometry (Flow)

1 µg/mL

-

Immunoprecipitation (IP)

1:10-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mustelid, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RW128

Immunogen

Fusion protein containing residues 1572-2371 of human p300. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p300,uniProtId:Q09472-1,ncbiNodeId:9606,antigenRange:1572-2371,antigenLength:2414,antigenImageFileName:MA1-16608_p300_Q09472-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16608_p300_Q09472-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568667

Product Specific Information

Suggested positive control: HeLa nuclear extract.

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.