Thermo Fisher Scientific p300 Monoclonal Antibody (RW128)
p300 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, Flow cytometry, IP에 사용 가능. 다양한 종(인간, 마우스 등)에 반응하며, Protein A로 정제된 액상 형태. HeLa 핵 추출물에서 양성 대조군으로 권장.
- 카탈로그번호
- MA116608
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250–1:500 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL | – |
| Flow Cytometry (Flow) | 1 µg/mL | – |
| Immunoprecipitation (IP) | 1:10–1:500 | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mustelid, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RW128 |
| Immunogen | Fusion protein containing residues 1572–2371 of human p300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568667 |
Product Specific Information
- Suggested positive control: HeLa nuclear extract.
Target Information
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein, cyclic-AMP responsive element binding protein) and, like CPB, can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A.
EP300 also acts as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), playing a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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