Thermo Fisher Scientific BRCA1 Monoclonal Antibody (KEN)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG3, kappa

Class

Monoclonal

Type

Antibody

Clone

KEN

Immunogen

Human BRCA1 containing residues 1314-1864. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BRCA1,uniProtId:P38398-1,ncbiNodeId:9606,antigenRange:1314-1863,antigenLength:1863,antigenImageFileName:MA1-16618_BRCA1_P38398-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16618_BRCA1_P38398-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• DyLight 488
• DyLight 650
• Request custom conjugation

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568467

Product Specific Information

Suggested positive control: U2OS and MCF-7 whole cell lysates.

Target Information

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.