Thermo Fisher Scientific Phospho-p53 (Thr55) Recombinant Rabbit Monoclonal Antibody (ARC1530)

Applications

• Western Blot (WB): 1:500–1:2,000
• ELISA: 1 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence: WFTED

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation. Upon stress, p53 activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.

p53 consists of:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 also plays roles in aging, oxidative stress, and memory-related processes. Normally expressed at low levels, it increases in transformed cells, contributing to malignancy. Mutations in TP53 disrupt DNA binding and tumor suppressor function, occurring both somatically and germline (e.g., Li-Fraumeni syndrome).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.