Thermo Fisher Scientific EB1/EB2/EB3 Monoclonal Antibody (KT65)

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:50

Product Specifications

Product Specific Information

The antibody detects a 30 kDa protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells.
It also detects EB1 by immunocytochemistry in HeLa and A431 cells.
The antibody may weakly detect endogenous EB2 and EB3 in some cells and tissues.

Target Information

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs).
These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes.
The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases, as well as various regulatory and adaptor proteins.

The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (alpha) and short (beta) isoforms.
These +TIPs contain an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP).
The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaction with EB1 and MTs.
A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins.
CLASPs are MT-stabilizing factors that localize to mitotic spindles, kinetochores, and the midbody.
CLASPs are important for cell division and may regulate cell migration and neuronal growth cone motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.