Thermo Fisher Scientific MAPRE3 Monoclonal Antibody (KT53)
MAPRE3 단백질(EB3)을 인식하는 Rat monoclonal antibody (Clone KT53). Western blot과 Immunocytochemistry에 적합하며, Human, Mouse, Rat 반응성. Protein G 정제, 액상 형태, 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KT53 |
| Immunogen | Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942695 |
Product Specific Information
The antibody detects a 32 kDa protein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue.
It also detects EB3 by immunocytochemistry in HeLa and A431 cells.
Target Information
EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli).
It binds to tubulin in vitro and microtubules in vivo. Northern blotting shows expression mainly in brain and to a lesser degree in muscle.
EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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