Thermo Fisher Scientific MAPRE3 Monoclonal Antibody (KT53)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KT53

Immunogen

Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2942695

Product Specific Information

The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.

Target Information

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.