Thermo Fisher Scientific Collagen Type II Polyclonal Antibody, HRP
HRP 결합형 Collagen Type II 폴리클로날 항체로, Western blot, IHC, ELISA, IP에 적합합니다. 인간 및 소 콜라겐 II에 반응하며, 다른 콜라겐 타입과의 교차반응이 거의 없습니다. 4°C 또는 -20°C에서 안정적으로 보관 가능합니다.
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Thermo Fisher Scientific Collagen Type II Polyclonal Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type II from adult human knee cartilage and bovine nasal cartilage |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 10 mg/mL BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with 0.05 mL of deionized water (or equivalent).
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Expiration date: one (1) year from date of restoration.
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.
This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V, and VI collagens.
Non-specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
COL2A1 encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye.
Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type.
Defects in processing chondrocalcin, a calcium-binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: 600-403-104_Collagen_II_P02458-2_Rabbit_PDP.jpeg, 600-403-104_Collagen_II_P02459-1_Rabbit_PDP.jpeg)
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