Thermo Fisher Scientific Collagen Type IV Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Bovine, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen IV,uniProtId:P02462-1,ncbiNodeId:9606,antigenRange:1-1669,antigenLength:1669,antigenImageFileName:600-403-106_Collagen_IV_P02462-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_P02462-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Collagen IV,uniProtId:P08572-1,ncbiNodeId:9606,antigenRange:1-1712,antigenLength:1712,antigenImageFileName:600-403-106_Collagen_IV_P08572-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_P08572-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Collagen IV,uniProtId:P53420-1,ncbiNodeId:9606,antigenRange:1-1690,antigenLength:1690,antigenImageFileName:600-403-106_Collagen_IV_P53420-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_P53420-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:Collagen IV,uniProtId:P29400-1,ncbiNodeId:9606,antigenRange:1-1685,antigenLength:1685,antigenImageFileName:600-403-106_Collagen_IV_P29400-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_P29400-1_Rabbit_PDP.jpeg,sortOrder:4},{targetFamily:Collagen IV,uniProtId:Q01955-1,ncbiNodeId:9606,antigenRange:1-1670,antigenLength:1670,antigenImageFileName:600-403-106_Collagen_IV_Q01955-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_Q01955-1_Rabbit_PDP.jpeg,sortOrder:5},{targetFamily:Collagen IV,uniProtId:Q29442-1,ncbiNodeId:9913,antigenRange:1-453,antigenLength:453,antigenImageFileName:600-403-106_Collagen_IV_Q29442-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_Q29442-1_Rabbit_PDP.jpeg,sortOrder:6},{targetFamily:Collagen IV,uniProtId:Q7SIB2-1,ncbiNodeId:9913,antigenRange:1-1669,antigenLength:1669,antigenImageFileName:600-403-106_Collagen_IV_Q7SIB2-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_Q7SIB2-1_Rabbit_PDP.jpeg,sortOrder:7},{targetFamily:Collagen IV,uniProtId:Q7SIB3-1,ncbiNodeId:9913,antigenRange:1-227,antigenLength:227,antigenImageFileName:600-403-106_Collagen_IV_Q7SIB3-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_Q7SIB3-1_Rabbit_PDP.jpeg,sortOrder:8},{targetFamily:Collagen IV,uniProtId:Q28084-1,ncbiNodeId:9913,antigenRange:1-471,antigenLength:471,antigenImageFileName:600-403-106_Collagen_IV_Q28084-1_Rabbit.svg,antigenImageFileNamePDP:600-403-106_Collagen_IV_Q28084-1_Rabbit_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 10mg/mL BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Target Information

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.