Thermo Fisher Scientific Collagen Type IV Polyclonal Antibody, HRP
HRP 결합형 Collagen Type IV 폴리클로날 항체로, Western blot, IHC, ELISA 등 다양한 응용에 적합. 인간 및 소 반응성. 높은 특이성과 낮은 교차 반응성. 친화 크로마토그래피로 정제된 고순도 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Collagen Type IV produced by repeated immunizations with Collagen Type IV from human and bovine placenta |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 10 mg/mL BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with 0.05 mL of deionized water (or equivalent).
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- Expiration date: 1 year from date of restoration.
Typically negligible cross-reactivity against other collagen types was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues.
This antibody reacts with most mammalian Type IV collagens and shows negligible cross-reactivity with Type I, II, III, V, and VI collagens. Non-specific cross-reaction with human serum proteins or non-collagen extracellular matrix proteins is negligible.
Target Information
Collagen IV is a major component of the basement membrane in tissues such as kidneys, lungs, and skin. It forms a network-like structure providing support and stability to surrounding tissues.
Composed of three alpha chains encoded by the COL4A1, COL4A2, and COL4A3 genes.
Mutations in these genes are associated with disorders like Alport syndrome and Goodpasture syndrome, affecting kidneys and lungs.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명 예시)
- 600-403-106_Collagen_IV_P02462-1_Rabbit.svg
- 600-403-106_Collagen_IV_P08572-1_Rabbit.svg
- 600-403-106_Collagen_IV_P53420-1_Rabbit.svg
- 600-403-106_Collagen_IV_P29400-1_Rabbit.svg
- 600-403-106_Collagen_IV_Q01955-1_Rabbit.svg
- 600-403-106_Collagen_IV_Q29442-1_Rabbit.svg
- 600-403-106_Collagen_IV_Q7SIB2-1_Rabbit.svg
- 600-403-106_Collagen_IV_Q7SIB3-1_Rabbit.svg
- 600-403-106_Collagen_IV_Q28084-1_Rabbit.svg
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