Thermo Fisher Scientific Collagen Type V Polyclonal Antibody, HRP

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• Restore with 0.05 mL of deionized water (or equivalent).
• For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
• Expiration date is one (1) year from date of restoration.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes, which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VI collagens. Non-specific cross-reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Target Information

Type I collagen is the fibrillar collagen found in most connective tissues and is the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with dermatofibrosarcoma protuberans, a skin tumor resulting from unregulated expression of the growth factor. Two transcripts, resulting from alternate polyadenylation signals, have been identified for this gene.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.