Thermo Fisher Scientific CHEK2 Monoclonal Antibody (OTI2F4), TrueMAB
CHEK2 단백질을 인식하는 Mouse Monoclonal Antibody로 Western Blot, ICC/IF, Flow Cytometry에 적합합니다. 인간, 개, 쥐에 반응하며, Lyophilized 형태로 제공됩니다. DNA 손상 체크포인트 경로 연구 및 암 관련 단백질 분석에 유용합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F4 |
| Immunogen | Full length human recombinant protein of human CHEK2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers. Upon DNA damage, CHK2 phosphorylates cell cycle regulators including tumor suppressor proteins such as BRCA1, aiding in survival after DNA damage. CHK2 is considered a tumor suppressor gene, with mutations linked to Li-Fraumeni syndrome and predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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