Thermo Fisher Scientific CUL4B Monoclonal Antibody (OTI7G10), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CUL4B is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes that mediate ubiquitination and subsequent proteasomal degradation of target proteins.
It functions as a scaffold protein, aiding catalysis by positioning substrates and ubiquitin-conjugating enzymes.
CUL4B plays roles in:

• Polyubiquitination of CDT1, histone H2A, H3, and H4 in response to DNA damage
• DNA repair and replication through interaction with DDB2
• Ubiquitination of cyclin E and regulation of G1 cell cycle progression
• Regulation of the mTOR pathway controlling cell growth and metabolism

CUL4B regulation of the mTORC1-mediated pathway depends on 26S proteasome function and interaction with MLST8.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.