Thermo Fisher Scientific Phospho-PPIG (Ser376) Polyclonal Antibody
Phospho-PPIG (Ser376) 폴리클로날 항체로 인산화된 PPIG 단백질 검출에 사용됩니다. Western blot, IHC, ICC/IF에 적합하며 사람, 마우스, 랫에서 반응합니다. 고순도 정제 및 안정한 PBS/glycerol 보존 용액 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PPIG (Accession Q13427), corresponding to amino acid residues around phosphorylated Ser376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816678 |
Product Specific Information
Antibody detects endogenous levels of PPIG only when phosphorylated at Ser376.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair, and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells, specifically responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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