Thermo Fisher Scientific Phospho-BRK (Tyr447) Polyclonal Antibody
BRK 단백질의 Tyr447 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin)에 적합. Human, Mouse, Rat 반응성. 고순도, 액상 형태로 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PTK6 (Accession Q13882), corresponding to amino acid residues around phosphorylated Tyr447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816680 |
Product Specific Information
This antibody detects endogenous levels of BRK only when phosphorylated at Tyr447.
Target Information
Breast tumor kinase (Brk) is a non-receptor protein tyrosine kinase expressed in over 60% of breast carcinoma tissue samples and breast tumor cell lines.
Brk shares 80% identity with Sik (Src-related intestinal kinase) and possesses SH3 and SH2 domains typical of non-receptor tyrosine kinases.
Low levels of Brk transcripts are found in some human breast tumor cell lines but not in normal breast tissue.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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