Thermo Fisher Scientific Phospho-BRK (Tyr447) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous levels of BRK only when phosphorylated at Tyr447.

Target Information

Breast tumor kinase (Brk) is a non-receptor protein tyrosine kinase expressed in over 60% of breast carcinoma tissue samples and breast tumor cell lines.
Brk shares 80% identity with Sik (Src-related intestinal kinase) and possesses SH3 and SH2 domains typical of non-receptor tyrosine kinases.
Low levels of Brk transcripts are found in some human breast tumor cell lines but not in normal breast tissue.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.