Thermo Fisher Scientific Phospho-PAK1/PAK2 (Thr423, Thr402) Polyclonal Antibody
PAK1 및 PAK2의 Thr423/Thr402 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot과 IHC(Paraffin)에서 검증됨. Human, Mouse, Rat 반응성. PBS/glycerol buffer에 보관, -20°C에서 안정적. 연구용 전용 제품.
- 카탈로그번호
- PA5105202
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PAK1 (Accession Q13153), corresponding to amino acid residues around phosphorylated Thr423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816675 |
Product Specific Information
This antibody detects endogenous levels of PAK1/PAK2 only when phosphorylated at Thr423.
Target Information
Three isoforms of serine/threonine kinases—designated αPAK (p68), βPAK (p65), and γPAK (p62)—exhibit high sequence homology with the S. cerevisiae kinase Ste20, which is involved in pheromone signaling.
The α, β, and γPAK isoforms specifically complex with Rac1 and Cdc42 in their active GTP-bound state, inhibiting intrinsic GTPase activity and leading to autophosphorylation.
There are eight autophosphorylation sites on γPAK, including Ser19, Ser141, and Thr402; phosphorylation of Ser141 and Thr402 correlates with γPAK activation.
Once phosphorylated, and with reduced affinity for Rac/Cdc42, the PAK isoforms dissociate from the complex to interact with downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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