Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), PerCP-eFluor 710, eBioscience
인간 CD137(4-1BB)을 인식하는 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 제품입니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, T세포 활성화 및 기억 T세포 생존 연구에 적합합니다. 488 nm 레이저에 의해 여기되어 710 nm에서 방출됩니다.
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), PerCP-eFluor 710, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B4 (4B4-1) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573715 |
Available Conjugate Formats:
- Alexa Fluor™ 700
- APC
- APC-eFluor™ 780
- FITC
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
Description:
This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), an inducible member of the TNFR family expressed on T cells, NK cells, dendritic cells, granulocytes, and mast cells. CD137 signaling recruits TRAF1 and TRAF2, contributing to T cell activation, survival of activated and CD8+ memory T cells, and regulation of myelopoiesis and dendritic cell development. It also promotes expansion of CD4+CD25+ T regulatory cells ex vivo. The ligand 4-1BBL is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
Applications Reported:
Validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on stimulated human peripheral blood cells.
- Recommended usage: 5 µL (0.125 µg) per test (in 100 µL final volume).
- Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited by the 488 nm blue laser.
- Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
- Ensure instrument compatibility before use.
Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Filtration:
0.2 µm post-manufacturing filtered.
Excitation/Emission: 488 nm / 710 nm
Laser: Blue Laser
Target Information
CD137 (4-1BB, TNFRSF9) is an inducible costimulatory molecule primarily expressed on activated T cells. Its ligand, 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling supports survival and expansion of activated and memory T cells, while regulating myelopoiesis and dendritic cell development. Soluble CD137 may act as a negative regulator in immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.